Build and execute DNA analysis workflows directly in your browser. Chain Needleman-Wunsch global alignment, 6-frame ORF scanning, PCR primer Tm calculation, and restriction digestions on an interactive canvas. Zero server dependencies.
Execute professional sequence operations inside standard browser runtimes. Built on pure TypeScript algorithms—zero remote API lag.
Performs global sequence alignment using dynamic programming, with customizable match, mismatch, and affine gap scoring matrices.
Scans forward and reverse complements across all six possible reading frames to map translation initiation and termination sites.
Determines forward/reverse primer pairs, computing primer-dimer risk and melting temperatures ($T_m$) via nearest-neighbor thermodynamics.
Digests sequence strands using a database of 200+ restriction endonucleases, indexing cut coordinates and fragment weight distribution.
Applies sliding window calculations to chart local G+C density ratios, highlighting CpG islands and genomic stability regions.
Calculates codon frequencies and Relative Synonymous Codon Usage index values to analyze host organism expression profile compatibility.
BioFlow organizes calculations into structured topological pipelines, maintaining downstream consistency in real-time.
Extract sequence payloads directly from standard FASTA text, FASTQ read streams, or annotated GenBank files without server-side processing.
Sorts analysis nodes into a Directed Acyclic Graph (DAG). Validates connections to prevent loops and structures execution flow.
Triggers pure-TypeScript executors sequentially. Changes to upstream outputs (like DNA mutations) prompt downstream recalculations.
Binds calculated values back to sequences. Highlights translation tracks, maps restriction cut lines, and projects alignments with overlay graphics.
Instantly deploy verified analytical topologies. Bypass setup overhead to inspect transcription, translation, and local restriction sites.
Load a FASTA file, find open reading frames, and analyze GC content.
Analyze restriction sites and design primers for cloning.
Translate a DNA sequence to protein and generate a report.
Align two sequences using the Needleman-Wunsch algorithm.
Load E. coli K-12 benchmark samples directly into local memory or start design workflows from scratch. No registration, no server constraints.
10,000 bp fragment from the E. coli K12 genome. Contains several known genes including lac operon region.
3,822 bp spike protein gene from SARS-CoV-2 (Wuhan-Hu-1 isolate).
5,600 bp fragment of the human BRCA1 gene (exon 11 region, known for breast cancer-associated mutations).